Description

Transcript assembly and quantification for RNA-Se

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, strandedness:‘forward’ ]

srbam

:file

Sorted and indexed bam file from short-read alignment.

.bam

lrbam

:file

Sorted and indexed bam file from long-read alignment.

.bam

mode

:string

StringTie mode (long-read-assembly, mix-read-assembly, expression-estimation, nascent-aware-assembly).

annotation_gtf

:file

Annotation gtf file (optional).

.gtf

Output

name:type
description
pattern

transcript_gtf

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, strandedness:‘forward’ ]

${prefix}.transcripts.gtf

:file

Output assembly (transcriptome)

.gtf

abundance

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, strandedness:‘forward’ ]

${prefix}.gene.abundance.txt

:file

Abundance for non-redundand set of transcripts

.txt

coverage_gtf

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, strandedness:‘forward’ ]

${prefix}.coverage.gtf

:file

Coverage for the sample

.gtf

ballgown

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, strandedness:‘forward’ ]

${prefix}.ballgown/*.ctab

:file

Ballgown output for the sample

.ctab

versions_stringtie

${task.process}

:string

The name of the process

stringtie

:string

The name of the tool

stringtie --version

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

stringtie

:string

The name of the tool

stringtie --version

:eval

The expression to obtain the version of the tool

Tools

stringtie2
MIT

Transcript assembly and quantification for RNA-Seq